On the evening of September 23, the ghetto was surrounded by the German Gendarmerie and the Ukrainian Auxiliary Police from Tuczyn (under the command of Ivan Stadnik) and from some other counties. On the following day, an SD unit from Równe arrived in the town, and the Germans and Ukrainian policemen entered the ghetto. They opened the gates, ordering the inmates to leave the ghetto in groups. When the latter offered resistance, the Germans and Ukrainian policemen began to shoot at them. The Jewish fighters returned fire, and the inmates torched the ghetto buildings, including the synagogues that the Germans had been using as warehouses. In the ensuing turmoil, Jews broke through the ghetto fence in several places. According to testimonies, as many as 2,000 Jewish inmates (including a large number of women and children) were able to flee into the nearby Pustomyty Forest. Most of the Jewish fighters stayed behind to keep the Germans occupied, and they either fell in battle or were captured and then shot. In the course of the fighting, two Ukrainian policemen and several Germans were killed or wounded.
The Germans and Ukrainian policemen soon launched a manhunt for the Jews who had escaped from the ghetto. Within three days, some 1,000 Jewish fugitives were recaptured, either in hiding or in the Pustomyty Forest, and most of them were killed in the Kotovskiy Grove north of Tuczyn, on the road from Tuczyn to the village of Reczyca. Upon reaching the murder site, the Jews were forced to strip naked and hand over any valuables that they still had with them. They were then driven into the pit in groups of several individuals, ordered to line up inside it, and shot by SD men, who used machine guns. As soon as one group of victims had been shot, another would be forced into the pit, ordered to lie down atop the bodies of their predecessors, and shot in the same manner. According to a testimony, the victims would occasionally be positioned on the lip of the pit with their backs to it, and then shot from behind. According to another testimony, a group of 30 Jews from the neighboring town of Hoszcza were also shot at this site.
Richter, the German commandant (senior official) of the Tuczyn Region, was in charge of this shooting action.