On July 26, 1941 the Germans ordered all the Jews of Biržai to leave their homes and move to a ghetto that was set up on a few streets around the synagogue and beit midrash.
Jewish men were taken from their homes to the Jewish cemetery and to other places in town and shot.
According to the Soviet Extraordinary Committee for the Examination of Nazi Atrocities, 30 bodies of Jewish victims were found in the cemetery, most of these victims had been shot in the head.