Transport No. 27 left Aspangbahnhof in Vienna (Wien) on April 27, 1942 at 7:11 pm and arrived in Wlodawa on April 30 at 6:00 am. This transport, whose original destination had been Izbica, consisted of 1000 Jews, among them 367 persons older than 61. The average age of the transport was 53 years. The train followed a route that went through Nordbahnhof in Vienna, Lundenburg (Breclav), Brno (Bruenn), Boehmisch-Truebau (Ceska Trebova), Wildenschwert (Usti nad Orlici), Mittelwald (Stredolesi), Glatz (Klodzko), Kamenz (Kamenec), Neisse (Nysa), Oppeln (Opole), Vosswalde (Fosowskie), Tschenstochau (Czestochowa), Kielce, Radom, Deblin, Lublin, Helm (Chelm) and Wlodawa. First Lieutenant Peter Johann and fifteen armed policemen from the Uniformed Police (Schupo) guarded the train throughout its journey.
The transport arrived at the Wlodawa rail station, which was located six kilometers from the town and seven kilometers from the Sobibor extermination camp, on April 29 at 23:00 after a 52-hour journey. Under the watchful eyes of policemen, the Jewish deportees had to make their way on foot from the rail station to the town. They were then transported to camps and ghettos in the vicinity. During June and October 1942, they were murdered in the Sobibor and Belzec extermination camps.